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PostPosted: Fri May 29, 2015 1:52 pm 
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Joined: Wed May 27, 2015 10:20 am
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The below Dr. Moore webpage seems to have been discontinued and I wish to save its data here.


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Osage Orange

Maclura pomifera

Osage Orange has traditionally been used as a treatment for cancer by Native American and folk herbalists. It is a botanical medicine encompassing unique constituents that exhibits anti-cancer activity with promising potential. A new class of lectins has been isolated from Osage Orange- prenylated isoflavones. The prenylated isoflavones are pomiferin and osajin.

Osage Orange has a strong resonance with the Doctrine of Signatures. Osage Orange has been recognized due to its shape which resembles a large tumor or fibroid. Its orange-red colored bark is associated with the color of blood, therefore it has been used traditionally in blood cleansing and blood movement. Utilitarian uses include the making of traditional bows. It is sometimes referred to by one of it's common names, Bow D'arc, which means wood of the bow. It is considered one of the best bow woods and is ranked among the Yew and the Pau D'arco, which are two of the top woods used in the construction of bows as well as anti-cancer botanicals. Osage Orange is an active ingredient in the formula Three Bows prescribed to treat cancer.

Constituents:

Maclura pomifera agglutinin (MPA) is a lectin/mitogen compound.
Macluralisin is a serin proteinase.
Pomiferin is a prenylated isoflavone, which acts as an antioxidant by inhibiting cytochrome P-450 enzymes, and inhibits LDL oxidation, a key process that is currently believed to play a central role in arteriosclerosis.
Osajin is also a prenylated isoflavone.
Phytoestrogen used in cancer and cardiovascular disease treatment.
Tetraphydroxystilbene is an anti-fungal agent.

Maclura pomifera agglutinin (MPA):

Highly specific for the tumor antigen Gal b1, 3 Gal Nac, a cell marker on some tumors. This is a core structure of the O-linked type of glycopeptide.
Has been used in immunology as erythroagglutinin, as markers in cell differentiation, in cancer biology as labels of carcinoma cells, in glycoprotein purification, and as probes in developmental biology.
It has shown to be detrimental to growth of larvae and to block HIV infection.
sequence of MPA, specifically for CHO and its lack of metal ions and disulfide bonds, all indicate it to be a member of a new lectin family.
It has a high affinity and specificity to bind tumor antigens on the surface of carcinoma cells from which 85% of cancer develops.

The Role of Lectins in Disease Treatment:

From the Latin word legere, the word lectin means "to bind" or "to pick and choose".
Lectins are non-immunologic protein-polysaccharide molecules having a strong binding affinity for the complex carbohydrates, which are abundant on cell surfaces.
Forming an irreversible covalent bond, lectins bind in a manner similar to antibodies.
Galactose/ N-acetylgalactose-amine (Gal/GalNAc) lectin on the macrophage surface is involved with the uptake and recognition of tumor cells.
Concentrations of lectin needed to produce a therapeutic effect are on the order of microgram doses.

Dosage:


Take 10 drops twice daily.

References:

Biou V., et al. (1995-96) ESRF Annual Report.
TA (1998) The role of lectins in the formation of disease and their potential use in treatment. The Journal of Naturopathic Medicine, v 8 no 2:25-34
Lee X & Biesterfeldt J (1997) Structure Determination of Maclura Pomifera Agglutinin: A Member of a New Lectin Family.
Pislariu C., et al. (2001) Detection and biological activity of phytoestrogens in wild and dietary plants. Plant Biology.
Rudenskaya GN., et al. (1995) Macluralism - a serin proteinase from fruits of Maclura pomifera. Planta, v 196: 174-9.
Rummel J (2001) Isolation and characterization of prenylated isoflavones from Maclura pomifera. Poster and Abstract.

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PostPosted: Fri May 29, 2015 1:53 pm 
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2,4,3′,5′-tetramethoxystilbene (TMS) is found in the Osage orange plant. The below found this compound to be a tumor inhibitor:


Quote:
Cancer Res November 15, 2001 61; 8164


Human cytochrome P450 (P450) 1B1 is found mainly in extrahepatic tissues and is overexpressed in a variety of human tumors. Metabolic activation of 17β-estradiol (E2) to 4-hydroxy E2 by P450 1B1 has been postulated to be a factor in mammary carcinogenesis. The inhibition of recombinant human P450 1B1 by 2,4,3′,5′-tetramethoxystilbene (TMS) was investigated using either bacterial membranes from a human P450/NADPH-P450 reductase bicistronic expression system or using purified enzymes. TMS showed potent and selective inhibition of the ethoxyresorufin O-deethylation (EROD) activity of P450 1B1 with an IC50 value of 6 nm. TMS exhibited 50-fold selectivity for P450 1B1 over P450 1A1 (IC50 = 300 nm) and 500-fold selectivity for P450 1B1 over P450 1A2 (IC50 = 3 μm). The inhibitory effects of TMS on EROD activity of human liver microsomes were determined. TMS inhibited EROD activity of human liver microsomes at the same concentration as with recombinant human P450 1A2. TMS also strongly inhibited 4- and 2-hydroxylation of E2 by P450 1B1-expressing membranes or purified P450 1B1. TMS was a competitive inhibitor of P450 1B1 with a Ki of 3 nm. The inhibition by TMS was not mechanism-based, and the loss of activity was not blocked by the trapping agents glutathione, N-acetylcysteine, or dithiothreitol. Using purified histidine-tagged P450 1B1, the binding kinetic analysis was performed with TMS, yielding a Kd of 3 μm. The activation of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline in an Escherichia coli lac-based mutagenicity tester system containing functional human P450 1B1 was strongly inhibited by TMS. Our results indicate that TMS is a very selective and potent competitive inhibitor of P450 1B1. TMS is selective for inhibiting P450 1B1 among other human P450s including 1A1, 1A2, and 3A4 and warrants consideration as a candidate for preventing mammary tumor formation by E2 in humans.

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