The Spitbox

DMSO, Vegetable oil, ozone
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Author:  DougA. [ Fri Mar 27, 2020 12:35 pm ]
Post subject:  DMSO, Vegetable oil, ozone

An interesting patent for formulating an ozonated oil with DMSO:


Method of preparing the composition According to this alternative method 25 ml vegetable oil and 25 ml DMSO were mixed into a 50:50 v/v mixture. The mixture was ozonized for 20 minutes under controlled flow and the reaction was monitored by detecting the presence of MSM in the mixture. The working conditions, flow and ozonization time were conveniently standardized and optimized taking as a reference the appearance of the MSM preferably in an amount of between 1% and 10%. The reaction was permitted to stop with the MSM in the mentioned values.

Antimicrobic Activity In Vi tro This test was carried out to compare the antimicrobic activity in vitro of the inventive composition and the vegetal oil alone. The following dilutions in triplicate were prepared, that is samples (ozonized vegetal oil and fluids of the invention) in nutritive broth: 5%, 2.5%, 0.5%, 0.25%, 0.1% and 0.01% w/v. The broth was placed in tubes and each tube was inoculated with 6 107 CFU (colonies forming units) of Staphylococcus aureus and incubated for 24 hrs . at 37 C° . After incubation a subculture of each dilution was carried out in agar tripticasa-soy. Table 1 shows the obtained results. NOTE: The tests have been carried in laboratory with multiple replicas, with the results herein shown being an average of the results obtained with the replicas.

Table 1 Concentration Ozonized Oil Composition of the % w/v Invention 1 2 3 1 2 3 0.01 +++ +++ +++ +++ +++ +++ 0.10 +++ +++ +++ +++ +++ +++ 0.25 +++ +++ +++ +++ +++ +++ 0.50 +++ +++ +++ + + + 2.50 ++ ++ ++ + + + 5.00 + + + - - - (+++) Abundant development (++) Medium development (+) Light development (-) No development


The above results clearly show that the antimicrobial activity of the inventive composition (fluid) is higher than the ozonized oil employed alone. EXAMPLE 3: Antimycotic Activity In Vi tro This test was carried out to compare the antimycotic power of the present composition and the one of an ozonized oil. Table 2 shows the activity of the inventive composition and a vegetal oil by separate over several species of fungus and yeast such as Penicillium, Aspergillus, Fusarium, and Candida Albicans . In each case all the microorganisms were treated with ozonized oil and the inventive composition comprising ozonized oil, DMSO and MSM.

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